DNA

Part:BBa_K2429016

Designed by: Adil Yusuf   Group: iGEM17_MIT   (2017-10-14)


mKate-FF4 Reporter

This is a 2-exon split mKate reporter construct and has an intron that contains 3 microRNA sites for FF4. These sites where not used in our experiments

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
    Illegal BamHI site found at 1
    Illegal XhoI site found at 437
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 540
    Illegal EcoRI site found at 1157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1062
    Illegal SapI.rc site found at 55


[edit]
Categories
Parameters
None